rnase inhibitor (New England Biolabs)
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rnase inhibitor
Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnase+inhibitor/RNase+Inhibitor%2C+Murine/pmc13693657-183-54-58
Average 99 stars, based on 3321 article reviews
Rnase Inhibitor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnase+inhibitor/RNase+Inhibitor%2C+Murine/pmc13693657-183-54-58
Average 99 stars, based on 3321 article reviews
rnase inhibitor - by Bioz Stars,
2026-11
99/100 stars
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In Vitro:Article Title: Structural basis for start codon selection by eIF5-mimic proteins Article Snippet: .. In vitro transcription reactions were carried out using 4 μg of purified DNA templates and purified recombinant T7 polymerase in transcription buffer [166 mM Hepes-KOH; (pH 7.5); 20 mM MgCl 2 ; 40 mM DTT; 2 mM spermidine; 25 mM each of adenosine 5′-triphosphate (ATP), guanosine 5′-triphosphate (GTP), cytidine 5′-triphosphate, and uridine 5′-triphosphate; and Purification:Article Title: Structural basis for start codon selection by eIF5-mimic proteins Article Snippet: .. In vitro transcription reactions were carried out using 4 μg of purified DNA templates and purified recombinant T7 polymerase in transcription buffer [166 mM Hepes-KOH; (pH 7.5); 20 mM MgCl 2 ; 40 mM DTT; 2 mM spermidine; 25 mM each of adenosine 5′-triphosphate (ATP), guanosine 5′-triphosphate (GTP), cytidine 5′-triphosphate, and uridine 5′-triphosphate; and Recombinant:Article Title: Structural basis for start codon selection by eIF5-mimic proteins Article Snippet: .. In vitro transcription reactions were carried out using 4 μg of purified DNA templates and purified recombinant T7 polymerase in transcription buffer [166 mM Hepes-KOH; (pH 7.5); 20 mM MgCl 2 ; 40 mM DTT; 2 mM spermidine; 25 mM each of adenosine 5′-triphosphate (ATP), guanosine 5′-triphosphate (GTP), cytidine 5′-triphosphate, and uridine 5′-triphosphate; and Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Isolation:Article Title: Human-specific morphoregulatory signatures in basal radial glia characterise neocortex evolution. Article Snippet: Transcriptome libraries were prepared using the SmartSeq 2 protocol, modified from96. .. Isolated total RNA from 10,000 or 5000 cells was denatured for 3min at 72 °C in 4 μL hypotonic buffer (0.2% Triton X-100) in the presence of 2.4mM dNTPs, 240 nM dT-primer and 4 U CMC:Article Title: PUS7 pseudouridylation of 7SK orchestrates human somatic cell reprogramming into iPS cells. Article Snippet: .. The CMC-treated and untreated RNA samples were resuspended in 6.5 μl of water and incubated with 0.5 μl of Incubation:Article Title: PUS7 pseudouridylation of 7SK orchestrates human somatic cell reprogramming into iPS cells. Article Snippet: .. The CMC-treated and untreated RNA samples were resuspended in 6.5 μl of water and incubated with 0.5 μl of Article Title: Sub-stoichiometric modifications of Aplysia californica tRNAs and tRNA fragments revealed by integrating intact and bottom-up mass spectrometry Article Snippet: Aplysia tRNA Glu CUC and tRNA Lys UUU were isolated from small RNA fractions using biotinylated complementary DNA probes (IDT) and streptavidin-functionalized Sepharose beads (Cytiva). .. First, 100 μL small RNA (∼400-500 ng/μL), 4 μL DNA probe (10 μM), 2.6 μL 20× SSC buffer (3 M sodium chloride, 300 mM trisodium citrate, pH adjusted to 7.0 with HCl), and 1 μL Ligation:Article Title: PUS7 pseudouridylation of 7SK orchestrates human somatic cell reprogramming into iPS cells. Article Snippet: .. The CMC-treated and untreated RNA samples were resuspended in 6.5 μl of water and incubated with 0.5 μl of Reverse Transcription:Article Title: Impacts of different SETD1A mutations and genetic backgrounds on human induced pluripotent stem cell-derived hippocampal neurons Article Snippet: Then, 2 ml of 5x SMARTScribe RT buffer (TaKaRa), 0.5 ml of DTT (100 mM). .. 0.3 ml of MgCl2 (200 mM), 1 μl of dNTPs (10 mM), 1 μl of TSO primer (10 μM, 5’-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) 0.25 μl of Tissue:Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Organoids:Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Extraction:Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Saline:Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Staining:Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Software:Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Magnetic Cell Separation:Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Mass Spectrometry:Article Title: Protocol for mechanical nuclei dissociation and isolation from frozen tissue and organoids for single-nucleus RNA sequencing. Article Snippet: Note: If working with a tissue typically composed of a large number of cells (or more than 2 million cells), dilute the sample 1:10 in DNase/RNase-free water directly into the tube containing Trypan Blue prior to counting. .. Biological samples Mouse brain tissue C57BL/6 (hemisphere) This study N/A Human ESC-derived cerebral organoids* This study WIBR1 Chemicals, peptides, and recombinant proteins Anti-Nucleus MicroBeads Miltenyi Biotec Cat#130-132-997 Bovine serum albumin (BSA) Bio Basic Cat# A0903 DAPI Sigma-Aldrich Cat#D9542 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat#D8418 DNase I Solution STEMCELL Technologies Cat#07900 Hydrochloric acid Sigma-Aldrich Cat#H1758 Nuclei Extraction Buffer Miltenyi Biotec Cat#130-128-024 Phosphate-buffered saline (D-PBS) Sigma-Aldrich Cat#P4474 Polymerase Chain Reaction:Article Title: Sub-stoichiometric modifications of Aplysia californica tRNAs and tRNA fragments revealed by integrating intact and bottom-up mass spectrometry Article Snippet: Aplysia tRNA Glu CUC and tRNA Lys UUU were isolated from small RNA fractions using biotinylated complementary DNA probes (IDT) and streptavidin-functionalized Sepharose beads (Cytiva). .. First, 100 μL small RNA (∼400-500 ng/μL), 4 μL DNA probe (10 μM), 2.6 μL 20× SSC buffer (3 M sodium chloride, 300 mM trisodium citrate, pH adjusted to 7.0 with HCl), and 1 μL Suspension:Article Title: Sub-stoichiometric modifications of Aplysia californica tRNAs and tRNA fragments revealed by integrating intact and bottom-up mass spectrometry Article Snippet: Aplysia tRNA Glu CUC and tRNA Lys UUU were isolated from small RNA fractions using biotinylated complementary DNA probes (IDT) and streptavidin-functionalized Sepharose beads (Cytiva). .. First, 100 μL small RNA (∼400-500 ng/μL), 4 μL DNA probe (10 μM), 2.6 μL 20× SSC buffer (3 M sodium chloride, 300 mM trisodium citrate, pH adjusted to 7.0 with HCl), and 1 μL Gentle:Article Title: Sub-stoichiometric modifications of Aplysia californica tRNAs and tRNA fragments revealed by integrating intact and bottom-up mass spectrometry Article Snippet: Aplysia tRNA Glu CUC and tRNA Lys UUU were isolated from small RNA fractions using biotinylated complementary DNA probes (IDT) and streptavidin-functionalized Sepharose beads (Cytiva). .. First, 100 μL small RNA (∼400-500 ng/μL), 4 μL DNA probe (10 μM), 2.6 μL 20× SSC buffer (3 M sodium chloride, 300 mM trisodium citrate, pH adjusted to 7.0 with HCl), and 1 μL |